Journal: Nucleic Acids Research

Article Title: The DNA Data Bank of Japan launches a new resource, the DDBJ Omics Archive of functional genomics experiments

doi: 10.1093/nar/gkr994

Figure Lengend Snippet: List of large-scale data released by DDBJ from July 2010 to June 2011

Article Snippet: Moreover, DDBJ has released the following: Expressed sequence tags (EST) and genome survey sequences (GSS) of fission yeast ( Schizosaccharomyces pombe ) submitted by Osaka City University, Japan; the kale ( Brassica oleracea var. acephala ) EST submitted by Kirin Holdings Company, Ltd, Japan; Metagenome and GSS of an uncultivated thermophilic archaeon ( Candidatus Caldiarchaeum subterraneum ) submitted by Japan Agency for Marine-Earth Science and Technology, Japan; the biofuel crop ( Jatropha curcas ) genome submitted by Kazusa DNA Research Institute, Japan; the mouse ( Mus musculus domesticus ) GSS submitted by the RIKEN BioResource Center, Japan; GSS of Japanese rice ( Oryza sativa Japonica Group) and African rice ( O. glaberrima ) and full length cDNAs of domesticated barley ( Hordeum vulgare subsp. vulgare ) submitted by National Institute of Agrobiological Sciences, Japan; WGS derived from chromosome 3H of domesticated barley ( H. vulgare subsp. vulgare cv.

Techniques: Clone Assay

Journal: PLoS ONE

Article Title: Identification of Heat Responsive Genes in Brassica napus Siliques at the Seed-Filling Stage through Transcriptional Profiling

doi: 10.1371/journal.pone.0101914

Figure Lengend Snippet: Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and microarray data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.

Article Snippet: To identify genes responsive to heat stress in oilseed rape at the seed-filling stage, a Brassica 95k EST microarray (jointly developed by the John Innes Centre and Cogenics ) was used to profile the transcripts from both the SW and seeds separated from 20 DAF siliques.

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Microarray